NCT06484101

Functional Characterization of CD8+ T Cells After Supramaximal Exercise

Effekten av högintensiv träning på Vita Blodkroppar, Med Speciellt Fokus på Funktion Kopplad Till Att bekämpa Cancerceller

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 7 (actual)
• Sponsor: Karolinska Institutet · Academic / Other
• Sex: All
• Age: 20 Years – 36 Years
• Healthy volunteers: Accepted

Summary

Healthy human subjects perform supramaximal exercise with venous blood samples obtained before and up to 60 minutes after the exercise bout.

Detailed description

Healthy, human subjects perform 3 x 30 seconds of supramaximal exercise. Venous blood samples were obtained before and up to 60 minutes after the exercise bout. The blood was used for absolute count of lymphocytes and monocytes per volume blood, and for isolation of CD8+ T cells. It was also used for blood gas analysis. The CD8+ T cells were characterized using flow cytometry, and then cultured for 7 days with CD8+ activation beads. The cells were counted and analyzed for viability at day4 and day8. RNAseq was performed on CD8+ cells isolated before and directly after exercise. CD8+ T cells from Day 1 pre and post exercise and then after 7 days of culture, were analyzed for IFNgamma release using Elispot, and for cytotoxicity against HEK cancer cells in vitro. Flow cytometry of CD8+ T cells was performed on fresh CD8 T cells pre and post exercise, and then on day4 and day8 after culture. SeaHorse metabolic assay was done on Day1 CD8 T cells obtained pre and post exercise.

Conditions

• Exercise Induced CD8+ T Cell Recruitment and Characterization of Function and Phenotype of These Cells

Interventions

Type	Name	Description
PROCEDURE	Exercise	3 x 30 seconds of supramaximal exercise

Timeline

• Start date: 2023-03-31
• Primary completion: 2023-12-22
• Completion: 2024-02-14
• First posted: 2024-07-03
• Last updated: 2024-07-03

Locations

1 site across 1 country: Sweden