NCT06456086

Effect of Epstein-Barr Virus on Macrophage M2/M1 Migration and EphA2 Expression in Adverse Drug Reactions

• Status: Active Not Recruiting
• Phase: —
• Study type: Observational
• Enrollment: 27 (actual)
• Sponsor: First Affiliated Hospital of Kunming Medical University · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

This study aims to investigate the effect of EBV reactivation or EBV reactivation added with dexamethasone(DXM) in patients with adverse drug reactions(CDR) , through evaluating the levels on monocyte, macrophage M2/M1 and cytokines. To investigate whether expression of EBV receptors EphA2 could specifically influence on EBV activation in CDRs. We performed a prospective longitudinal study on the frequencies of Monocyte, Macrophage, M2/M1 and cytokines included IL-4,IL-13,TNF-α,IFN-γ,IFN-β, CXCL9 and CXCL10 after onset of MPE ,SJS/TEN and control groups. PBMCs collected from peripheral blood were cocultured with EBV or EBV with DXM. We next examined whether EBV or EBV with DXM could have a strong impact on the MOs, Mac, M2/M1 and cytokines and which cytokines could be crucial for the interaction between M2/M1 and EBV, by in vitro cocultures. Finally, EphA2 were detected to evaluate reactivation of EBV.

Conditions

• Adverse Drug Reactions

Interventions

Type	Name	Description
BIOLOGICAL	stimulation with EB virus	stimulation with EB virus

Timeline

• Start date: 2020-12-01
• Primary completion: 2024-03-31
• Completion: 2024-10-31
• First posted: 2024-06-13
• Last updated: 2024-06-13

Locations

1 site across 1 country: China