NCT06396910

TB and Sarcoidosis Granuloma

Immune Maping of Tuberculosis and Sarcoidosis Granuloma

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 20 (actual)
• Sponsor: Karolinska Institutet · Academic / Other
• Sex: All
• Age: 10 Years – 75 Years
• Healthy volunteers: Not accepted

Summary

Tuberculosis (TB) and sarcoidosis are both granulomatous diseases. Here we compared the immunological micro-environments of granulomas from TB and sarcoidosis patients using in situ sequencing (ISS) transcriptomic analysis and multiplexed immunolabelling of tissue sections.

Detailed description

Tuberculosis (TB) and sarcoidosis are diseases that are characterized by the formation of inflammatory structures called granulomas. TB is caused by infection with the bacteria Mycobacterium tuberculosis while sarcoidosis is a an inflammatory disease of unknown ethiology. We hypothesize that the localization of immune molecules in granulomas determines the the course of TB and sarcoidosis using in situ sequencing, a spatial transcriptomic technology and immunolabelling in biopsies from patients. In the project, we aim to answer the following questions: 1. How does the immune landscape differ in different TB granulomas (with/without necrosis, different histology)? 2. How does the immune landscape differ in acute (Lofgren's syndrome) and chronic (non-Lofgren's syndrome) sarcoidosis samples? 3. How does the immune landscape differ in histologically similar granulomas from TB and sarcoidosis patients? We will use in situ sequencing that can identify the exact location of 65 specific immune markers in the lung at the same time, thereby correlating defined immune landscapes with the histology, bacterial content and disease type.

Conditions

• Tuberculosis
• Sarcoidosis

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Spatial transcriptomics	Localize immune transcripts in the pulmonary lessions of sarcoidosis and tuberculosis patients. This is done in biopsies from pathological libraries.

Timeline

• Start date: 2021-09-01
• Primary completion: 2022-09-01
• Completion: 2023-07-01
• First posted: 2024-05-02
• Last updated: 2024-05-02

Locations

1 site across 1 country: Sweden