NCT06193668

Overfeeding Induced Fat-tissue Stimulation

Short-term High-fat Overnutrition Induces Insulin Resistance in White Adipose Tissue

• Status: Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 32 (estimated)
• Sponsor: German Diabetes Center · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

Type 2 diabetes is the most common metabolic disease worldwide, characterized by hyperglycemia, decreased whole body insulin sensitivity, and white adipose tissue (WAT) dysfunction. A key factor in its development is chronic overnutrition, usually with a high-fat diet (HFD), leading to disturbances of glucose and lipid metabolism. However, the mechanism of short-term HFD-induced tissue-specific insulin resistance remains poorly understood. This project aims to further unravel the underlying mechanisms of short-term HFD overnutrition-mediated WAT insulin resistance. The model described here corresponds to a randomized, single- blinded parallel-grouped trial, consisting of two interventions: a macronutrient-balanced diet and or a hypercaloric diet over three weeks in order to investigate differences in interorgan fatty acid and glucose metabolism between the studied groups. Based on recent studies, the hypothesis is that 21-day hypercaloric HFD induces WAT insulin resistance via a diacylglycerol, novel protein kinase C-insulin receptor signaling model in both fasting and insulin-stimulated states.

Conditions

• Lipid-induced Insulin Resistance

Interventions

Type	Name	Description
BEHAVIORAL	High-fat overnutrition	Overnutrition: 40% higher lipid consumption per day than required
BEHAVIORAL	Normocaloric macronutrient-balanced nutrition	Normocaloric, macronutrient-balanced nutrition: Calories requirement for weight maintenance \[(kcal/d) 55% carbohydrates, 15% proteins, 30% fat\]

Timeline

• Start date: 2024-01-31
• Primary completion: 2025-12-31
• Completion: 2030-12-31
• First posted: 2024-01-05
• Last updated: 2024-03-01

Locations

1 site across 1 country: Germany