NCT06065930

Adaptive Mechanisms In GRown up ObeSity Study (AMIGROS)

Mechanistic Studies in Human Subcutaneous Adipose Tissue

• Status: Recruiting
• Phase: —
• Study type: Observational
• Enrollment: 45 (estimated)
• Sponsor: Vastra Gotaland Region · Other Government
• Sex: All
• Age: 40 Years – 70 Years
• Healthy volunteers: Accepted

Summary

The investigator recently showed that the glycan-binding adipokine galectin-1 increased during overfeeding and that galectin-1 independently could predict type 2 diabetes. Further, the molecules that induce insulin release in the fasting state when blood glucose is normal remain elusive. It is possible that galectin-1 is involved in adaptive mechanisms in adipose tissue in obese subjects.

Detailed description

The investigator will define adaptive mechanisms in adipose tissue associated with galectin-1 in obese insulin-sensitive (Ob-IS) subjects compared with obese insulin-resistant (Ob-IR) subjects and lean healthy controls. Further, the investigator will study molecules secreted from adipose tissue that might trigger insulin secretion when blood glucose is normal. The investigator hypothesizes that Ob-IS subjects keep fatty acid levels normal through an adaptive response in adipose tissue that involves up-regulation of galectin-1 for dampening of immune cell activity and stimulation of lipolysis.

Conditions

• Obesity
• Type 2 Diabetes

Interventions

Type	Name	Description
DEVICE	Subcutaneous microdialysis	Group with Ob-IS participants, collection of abdominal subcutaneous interstitial dialysates after an overnight´s fast for later measurements of metabolites and peptides eg galectin-1. Group with Ob-IR participants, collection of abdominal subcutaneous interstitial dialysates after an overnight´s fast for later measurements of metabolites and peptides eg galectin-1. Group with Leans, collection of abdominal subcutaneous interstitial dialysates after an overnight´s fast for later measurements of metabolites and peptides eg galectin-1.

Timeline

• Start date: 2023-05-05
• Primary completion: 2025-12-31
• Completion: 2028-12-31
• First posted: 2023-10-04
• Last updated: 2023-10-04

Locations

1 site across 1 country: Sweden