NCT05954221

Red Cell Lysis Buffer Application Versus Conventional Sample Processing in EUS-FNB

Red Cell Lysis Buffer Application Versus Conventional Sample Processing in Endoscopic Ultrasonography (EUS)-Guided Biopsy for Solid Pancreatic Lesions

• Status: Unknown
• Phase: N/A
• Study type: Interventional
• Enrollment: 66 (estimated)
• Sponsor: Changhai Hospital · Academic / Other
• Sex: All
• Age: 18 Years – 80 Years
• Healthy volunteers: Not accepted

Summary

The goal of this clinical trial is to evaluate the application of red cell lysis buffer (RCLB) versus conventional sample processing in endoscopic ultrasonography (EUS)-guided biopsy for solid pancreatic lesions. The main questions it aims to answer are: whether the application of red cell lysis buffer improves histological tissue quality by decreasing blood contamination. Participants with solid pancreatic lesions who needs histological diagnosis will receive EUS-guided biopsy. The obtained specimens will be processed by RCLB and conventional formalin solution. Researchers will compare the blood contamination score of specimens in RCLB group with the conventional group see if the blood contamination can be improved.

Conditions

• Pancreatic Diseases

Interventions

Type	Name	Description
PROCEDURE	Red cell lysis buffer (RCLB) application	The tissue is lysed with 50% concentration of RCLB for 15 min on a shaking device. If the lysis is judged to be insufficient, it can be repeated one more time. Finally, the treated tissue will be fixed with 10% formalin.
PROCEDURE	Conventional processing	The tissue is fixed with 10% formalin.

Timeline

• Start date: 2023-09-15
• Primary completion: 2023-10-30
• Completion: 2023-12-31
• First posted: 2023-07-20
• Last updated: 2023-09-07

Locations

1 site across 1 country: China