Trials / Unknown
UnknownNCT05811546
Immunohistochemical Evaluation of DNA Methylation and Histone Modification in H. Pylori Associated Gastric Carcinoma
- Status
- Unknown
- Phase
- —
- Study type
- Observational
- Enrollment
- 68 (estimated)
- Sponsor
- Assiut University · Academic / Other
- Sex
- All
- Age
- 30 Years – 100 Years
- Healthy volunteers
- Not accepted
Summary
The goal of this cross-sectional study is to evaluate epigenetic modifications in gastric carcinogenesis, mainly in H. pylori gastritis vs gastric carcinoma. The main question\[s\] it aims to answer are: * How does H. pylori infection cause gastric cancer? * Can DNA methylation and other epigenetic changes predict and affect the development of gastric cancer? Formalin-fixed-paraffin-embedded tissue specimens will be obtained and reviewed for comparison between epigenetic changes in H. pylori gastritis and gastric adenocarcinoma.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| DIAGNOSTIC_TEST | Immunohistochemical staining for DNMT1 and EZH2 | Immunohistochemical staining for DNMT1 and EZH2 using rabbit monoclonal/polycolonal antibodies with immunoperoxidase staining will be used as a visualization method. Antibodies dilutions, antigen retrieval methods, and incubation time will all be conducted according to the manufacturer's instructions. |
Timeline
- Start date
- 2022-05-01
- Primary completion
- 2024-12-31
- Completion
- 2024-12-31
- First posted
- 2023-04-13
- Last updated
- 2023-04-13
Locations
1 site across 1 country: Egypt
Regulatory
- FDA-regulated device study
Source: ClinicalTrials.gov record NCT05811546. Inclusion in this directory is not an endorsement.