NCT05766293

ESWT on Human Gingival Fibroblasts

Application of Extracorporeal Shockwaves on Human Gingival Fibroblasts in Vitro Study

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 20 (actual)
• Sponsor: Dr Prabhuji MLV · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis. The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, these cells were assessed for Cell - Cell interaction and Cell Viability. In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed

Detailed description

BACKGROUND: Improved understanding of human biology at the molecular level, scientific and technologic advances are emerging aspects in dentistry. The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis. AIM: The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival tissue derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, fibroblast cells were assessed for Cell - Cell interaction and Cell Viability. MATERIALS AND METHODS: In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed using wound scratch assay which was used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay was used to determine Cell viability after exposure to shockwaves.

Conditions

• Periodontal Attachment Loss

Interventions

Type	Name	Description
DEVICE	extracorporeal shockwave device	Fibroblast cell lines (n=10) were subjected to Shockwaves from extracorporeal shockwave device
OTHER	Fibroblast cell lines not subjected to shockwaves from extracorporeal shockwave device	Fibroblast cell lines (n=10) without shockwave exposure

Timeline

• Start date: 2022-08-22
• Primary completion: 2022-11-17
• Completion: 2023-01-10
• First posted: 2023-03-13
• Last updated: 2023-03-13

Locations

1 site across 1 country: India