NCT05642767

Molecular Detection Of Efflux Pump and Virulence Factors Genes in Pseudomonas Aeruginosa

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 75 (estimated)
• Sponsor: Sohag University · Academic / Other
• Sex: All
• Age: 4 Weeks – 80 Years
• Healthy volunteers: Not accepted

Summary

Pseudomonas aeruginosa (PA) is a ubiquitous aerobic, non-fermentative Gram-negative rod that is widely associated with nosocomial pneumonia and can lead to severe illness with poor outcomes, particularly in critically ill people due to the ability of some strains to cause lung epithelial injury and spread into the circulation. 2 In the intensive care unit, PA infection is ranked among the top five causes of the bloodstream, pulmonary, surgical site, urinary tract, and soft tissue infections.

Detailed description

The pathogenesis of PA infections is multifactorial, and it is frequently complicated by the bacteria's intrinsic resistance to some antimicrobial agents such as sulfonamides, tetracyclines, and trimethoprim, as well as its ability to acquire or quickly develop resistance to major classes of antibiotics such as aminoglycosides, quinolones, B-lactams, and polymyxins (Bassetti et al., 2018). The efflux systems, which mediate the expulsion of antibiotics out of the cell shortly after entry, the production of enzymes to inactivate antibiotics, and the decrease in permeability across the cell wall are some mechanisms used by PA to develop antimicrobial resistance (Meletis \& Bagkeri, 2013). PA possesses a large number of virulence factors that play a significant role in pathogenesis and the determination of infection severity. These virulence factors act alone or in synergy with each other to cause tissue damage, necrosis, and cell death. Among the virulence factors of PA, the major determinants of virulence are the type III secretion system (T3SS) and quorum sensing (cell-to-cell signaling system). The T3SS is a needle-like complex, also known as the injectisome, that enables a bacterium to deliver different effector proteins such as ExoS, ExoT, ExoU, and ExoY across the membrane into a host cell, altering host cell functions and increasing bacterial survival rates ( Horna G and, Ruiz J, 2021). In this study, we aimed to evaluate the prevalence of antibiotic resistance caused by the presence of Efflux genes and some virulence factors in Pseudomonas aeruginosa from clinical isolates.

Conditions

• Pseudomonas Aeruginosa

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	culture on cetrimide agar	Samples will be inoculated on cetrimide agar using the plating out technique.
DIAGNOSTIC_TEST	Staining with Gram stain	colonies on cetrimide agar will be spread on glass slide and stained by gram stain
DIAGNOSTIC_TEST	Antibiotic sensitivity testing	Antibiotic sensitivity testing will be done by disc diffusion method according to CLSI
DIAGNOSTIC_TEST	Molecular detection to efflux genes and some virulence genes	Molecular detection to efflux genes and some virulence genes by conventional PCR

Timeline

• Start date: 2022-12-01
• Primary completion: 2023-03-30
• Completion: 2023-05-01
• First posted: 2022-12-08
• Last updated: 2023-04-12

Locations

1 site across 1 country: Egypt