NCT05446610

Investigating Effects in Intestinal Permeability of Rich in Beta-glucans Pleurotus Eryngii Mushrooms Fermentation Supernatants: an Ex-vivo Study

Development of a Novel Functional Food Enriched With Beta-glucans Isolated From Edible Mushrooms of Greek Habitats

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 10 (actual)
• Sponsor: Örebro University, Sweden · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

The aim of the present study was to investigate the ability of Pleurotus eryngii mushrooms fermentation products (FS) to counteract induced intestinal hyperpermeability in human colonic tissues in an ex vivo system.

Detailed description

Collection of colon biopsies through sigmoidoscopy procedure will take place and the collected biopsies will be mounted in Ussing Chambers. Already collected fermentation supernatants will be added to the mucosal side of the biopsy together with a stressor and two permeability markers, in order to investigate the effects of the fibre fractions on both paracellular and transcellular permeability.

Conditions

• Healthy

Interventions

Type	Name	Description
OTHER	Fermented product of Pleurotus eryngii mushroom	I Stimulation of human colonic biopsies with the fermented product of Pleurotus eryngii mushroom

Timeline

• Start date: 2020-03-17
• Primary completion: 2020-09-18
• Completion: 2022-06-17
• First posted: 2022-07-06
• Last updated: 2022-07-06

Locations

1 site across 1 country: Sweden