NCT05443282

Ovarian Reserve and Matrix Metalloproteinases

Serum Prolidase Activity in Primary Ovarian Insufficiency

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 133 (actual)
• Sponsor: Ankara City Hospital Bilkent · Academic / Other
• Sex: Female
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

Primary Ovarian Insufficiency (POI) is dysfunction of ovaries before 40 years of age. It has been reported that genetic factors, endocrine disorders, previous pelvic surgeries, chemotherapy and radiotherapy, and autoimmune mechanisms play a role in 10%, however the etiology has not been clarified in 90% of the cases. Oxidative stress and autoimmunity has been shown to be responsible in the etiopathogenesis of POI. Prolidase is an imidodipeptidase. Prolidase was shown to be related with oxidative stress and autoimmune diseases in previous studies. In addition, it is thought that the level of prolidase plays a role both in the etiopathogenesis and progression of diseases. This study aims to investigate the level of prolidase enzyme in women with POI.

Detailed description

Primary Ovarian Insufficiency (POI) is dysfunction of ovaries before 40 years of age. It has been reported that genetic factors, endocrine disorders, previous pelvic surgeries, chemotherapy and radiotherapy, and autoimmune mechanisms play a role in 10%, however the etiology has not been clarified in 90% of the cases that are diagnosed as idiopathic POI. Recent studies concentrated on the role of immune mechanisms in idiopathic POI. Prolidase is a matrix metalloproteinase that plays a role in various metabolic processes and is responsible for the destruction of dipeptides containing proline and hydroxyproline. The prolidase has been shown to be related with oxidative stress and autoimmune diseases in previous studies. Prolidase was shown to be a diagnostic and prognostic value in a number of diseases. Proline and hydroxyproline are metabolites produced through the prolidase activity. Proline itself is also considered as a kind of metabolic stress indicator. On the other hand, free proline in the medium is known to inhibit the prolidase enzyme activity. Therefore, simultaneous measurement of proline and hydroxyproline levels in the medium enables to more accurately calculate the prolidase activity. This study aim to analyze the prolidase level in POI patients compared to healthy menstruating women. Besides the prolidase activity, we also aim to measure proline and hydroxyproline levels in order to provide the calibration for accurate results in terms of prolidase activity.

Conditions

• Oxidative Stress
• Ovarian Insufficiency, Primary
• Ovarian Failure, Premature

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Prolidase Level	Measurement of venous blood serum level of prolidase enzyme level by ELISA method.
DIAGNOSTIC_TEST	Follicle Stimulating Hormone Level	Measurement of venous blood serum level of follicle stimulating hormone level.
DIAGNOSTIC_TEST	Estradiol Level	Measurement of venous blood serum level of estradiol hormone level.
DIAGNOSTIC_TEST	Proline Level	Measurement of venous blood serum level of proline level.
DIAGNOSTIC_TEST	Hydroxyproline Level	Measurement of venous blood serum level of hydroxyproline level.
DIAGNOSTIC_TEST	Anti-mullerian Hormone Level	Measurement of venous blood serum level of anti-mullerian hormone level.
DIAGNOSTIC_TEST	Antral Follicle Count	Ultrasonographic evaluation of antral follicle count to determine ovarian reserve.
DIAGNOSTIC_TEST	Prolactine Level	Measurement of venous blood serum prolactine hormone level.
DIAGNOSTIC_TEST	Thyroid Stimulating Hormone Level	Measurement of venous blood serum thyroid stimulating hormone level.

Timeline

• Start date: 2021-08-10
• Primary completion: 2022-02-12
• Completion: 2022-03-07
• First posted: 2022-07-05
• Last updated: 2022-07-05

Locations

1 site across 1 country: Turkey (Türkiye)