NCT05421793

Prebiotic and Anti-inflammatory Effects of a Pectin Polysaccharide

Towards a Food Ingredient Clinically Proven to Benefit Gut Health: Novel RG-I Variants

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 17 (actual)
• Sponsor: Örebro University, Sweden · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

The aim of this study is to determine, quantify and understand the potential prebiotic and anti-inflammatory effects of the pecticpolysaccharide rhamnogalacturonan I (RG-I). The effects of these dietary fibre fractions on barrier function will also be investigated.

Detailed description

The dietary fibre fractions from RG-I will be tested first for their prebiotic potential. Batch culture fermentation systems containing basal growth medium will be inoculated with faecal homogenates obtained from different time points (Task I) and will be used for analyzing microbiota composition and microbiota-associated metabolites. Subsequently, the collection of colon biopsies through sigmoidoscopy procedure of the same subjects will take place and the collected biopsies will be mounted in Ussing Chambers. Supernatants collected from Task I will be added to the mucosal side of the biopsy together with a stressor and two permeability markers, in order to investigate the effects of the fibre fractions on both paracellular and transcellular permeability.Throughout the study subjects will complete questionnaires related to their gastrointestinal health and dietary habits

Conditions

• Healthy

Interventions

Type	Name	Description
OTHER	Fermented product of carrot derived rhamnogalacturonan I	Stimulation of human colonic biopsies with the fermented product of carrot derived rhamnogalacturonan I

Timeline

• Start date: 2022-06-06
• Primary completion: 2023-07-30
• Completion: 2023-08-30
• First posted: 2022-06-16
• Last updated: 2023-09-21

Locations

1 site across 1 country: Sweden