NCT05304325

Adipose Tissue Pre and Post Bariatric Surgery

Evaluation of Adipose Tissue Pre and Post Bariatric Surgery

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 98 (actual)
• Sponsor: Institut de Recherches Cliniques de Montreal · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

Published data suggest that inflammation and fibrosis of adipose tissue could be factors favoring the development of insulin resistance in obese individuals and that a decrease in the activity of the AMP-activity kinase protein (AMPK) could lead to these dysfunctions. However, very few data are available in humans. There is also growing interest in persistent organic pollutants (POPs) as a cardiometabolic and type 2 diabetes (T2D) risk factor. There is some evidence to suggest that POPs directly contribute to lipid metabolism dysfunction and insulin resistance. Additionally, POPs are stocked in adipose tissue. The accumulation of POPs in adipose tissue therefore limits their bioavailability to other organs, thus reducing their systemic toxicity. It has been observed that a large amplitude weight loss leads to a significant increase in POPs in the blood. The goal of this project is to identify adipose tissue factors/dysfunctions that contribute to insulin resistance and type 2 diabetes associated with obesity in humans and thus raise avenues for screening and treatment of these metabolic complications. More specifically, the objectives are: * To study the relationship between AMPK, fibrosis and inflammation of adipose tissue and their role in the development of insulin resistance and T2D associated with obesity; * To examine the relationship between POPs and the cardiometabolic profile.

Conditions

• Obesity

Interventions

Type	Name	Description
PROCEDURE	Bariatric surgery	Sleeve gastrectomy
OTHER	Blood sampling	Blood samples will be collected to measure glucose, insulin, albumin, uric acid, HbA1c, lipids, hepatic enzymes, TSH, hs-CRP, complete blood cell counts and persistant organic pollutants
OTHER	Blood pressure	Systolic and diastolic blood pressure will be measured.
OTHER	Anthropometric measurements	Body weight, height and waist circumference will be measured.
OTHER	Body composition	Body composition will be evaluated by dual-energy X-ray absorptiometry.
OTHER	Resting metabolic rate	Resting metabolic rate will be measured by indirect calorimetry.
OTHER	Food intake	Food intake, energy intake and food quality will be evaluated using the food frequence questionnaire.
PROCEDURE	Adipose tissue biopsy	A sample of 3-5g of adipose tissue will be collected under local anesthesia by needle aspiration in the periumbilical region.

Timeline

• Start date: 2012-04-25
• Primary completion: 2022-07-31
• Completion: 2022-09-30
• First posted: 2022-03-31
• Last updated: 2022-03-31

Locations

1 site across 1 country: Canada