NCT05170360

Evaluation of Some Risk Factors Associated With Colorectal Cancer

Evaluation of Some Risk Factors Associated With Colorectal Cancer and Their Correlation With KRAS and BRAF Genes Mutations

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 100 (estimated)
• Sponsor: Assiut University · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: —

Summary

1. Evaluation of some risk factors (e.g. smoking, heavy metal exposure, and organophosphorus exposure) on CRC. 2. Estimation of KRAS and BRAF genes mutations associated with CRC. 3. Association between different risk factors and gene mutations on different types of CRC.

Detailed description

* Colorectal cancer (CRC) ranks as the third most common cancer globally and second in terms of mortality (Bray et al., 2018). CRC is the 7th commonest cancer in Egypt, representing 3.47% of male cancers and 3% of female cancers (Metwally et al.,2018). * Studies have provided that rising CRC rates are associated with increased alcohol use, physical inactivity, high intake of fat, red and processed meat, and processed foods; and low intake of fiber (Zhou and Rifkin, 2021). * Smoking is an established risk factor for colorectal adenomas as well as CRC incidence and mortality, which suggests that it may affect the prognosis of CRC patients as well (Walter et al., 2014). * Heavy metals are natural components of the earth's crust, which, if released into the environment, can persist for many years (Wu et al., 2016). The most toxic elements are arsenic, cadmium, chromium, copper, mercury, and lead with the majority of them being classified as certainly or probably carcinogenic (Forte et al., 2020). * Pesticides that are applied to farms can remain in the environment for longer than intended (Sjerps et al., 2019). Pesticides increase the risk of cancer through a variety of mechanisms including genotoxicity, epigenetic effects, hormonal action, and immunotoxicity (Lyons and Watterson, 2010). * Of the key genes of CRC, KRAS mutations are the most widely known, as they are mainly localized in codons 12 and 13, which were among the first linked to the pathogenesis of colon cancer (Kudryavtseva et al., 2016). BRAF is an oncogene located on chromosome 7 for which gain of function mutations in multiple solid tumors, such as colon cancer (Frisone et al., 2020).

Conditions

• Colorectal Neoplasms

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Assessment of smoking ,heavy metal, organophosphorus exposure and d) Mutational analysis of the KRAS and BRAF genes:	1. Assessment of smoking exposure: Serum cotinine will be measured by using serum cotinine ELISA kits 2. Assessment of heavy metal exposure: Lead and cadmium will be measured using Graphite Tube Atomizer. 3. Assessment of organophosphorus exposure: By measuring pseudocholinesterase level by spectrophotometer 4. Mutational analysis of the KRAS and BRAF genes: the tissue samples will be used to determine the mutation in k-ras and BRAF genes by RCR

Timeline

• Start date: 2021-12-01
• Primary completion: 2023-06-01
• Completion: 2023-12-01
• First posted: 2021-12-28
• Last updated: 2021-12-28