NCT05113225

Sperm Micro-swim up Technique; Process and Select in the Same Dish: a Sibling-oocyte-split Design

Sperm Micro-swim up; a Novel Technique to Process and Select in the Same Dish:a Sibling-oocyte-split Design)

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 156 (actual)
• Sponsor: Royal Fertility Center, Egypt · Academic / Other
• Sex: Female
• Age: 18 Years – 40 Years
• Healthy volunteers: Not accepted

Summary

A sibling-Oocyte-split design will be conducted on oocytes before denudation and divided into two groups : control group (MII oocytes will be injected using sperm processed by Density gradient method) and sample group ( MII oocytes will be injected by sperm processed using micro-swim up technique)

Detailed description

In This study investigators compare a novel sperm processing and selection method which is (fast, cheap and safe) with the well known processing method of sperm "Density gradient (DG)" The dishes will be prepared with droplets of handling medium for oocytes (droplet volume 10 μL), and an H pattern using PVP will be produced, finally, all the droplets will be overlaid with mineral oil. Control group : Oocytes will be injected using sperm after processing with DG and will be selected from control PVP microdroplets Sample group: Sperm will be directly loaded in specific ICSI dishes where is the PVP microdroplets is prepared on "H" Pattern and will be selected from the far point from the deposition point and will be injected into the oocytes from the sample group All injected MII oocytes from both groups will then be cultured, with each group demarcated in separate place in the culture dish then according to the randomisation embryo transfer will be selected from one of both groups then the quality of the embryos will be reported and the pregnancy rate

Conditions

• Assisted Reproductive Technology
• Infertility

Interventions

Type	Name	Description
PROCEDURE	Micro-swim up technique	processing and selection of the sperm for ICSI in the same dish

Timeline

• Start date: 2021-10-10
• Primary completion: 2021-12-25
• Completion: 2021-12-30
• First posted: 2021-11-09
• Last updated: 2022-06-23

Locations

1 site across 1 country: Egypt