NCT05111717

The Effect of Desfluran On Global Dna Methylation

The Effect of Desfluran Administered in General Anesthesia On Global Dna Methylation

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 40 (actual)
• Sponsor: Muğla Sıtkı Koçman University · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Not accepted

Summary

The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.

Detailed description

Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.

Conditions

• DNA Damage

Interventions

Type	Name	Description
DRUG	Desflurane	Peripheral venous blood samplesof patients will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). Anesthesia maintenance will be provided with 40% O₂ and minimum alveolar concentration (MAC) in 60% air with 6% desflurane. DNA will be obtained from blood samples with the help of DNA isolation kit, and global DNA methylation analysis will performed by ELISA method.

Timeline

• Start date: 2021-11-17
• Primary completion: 2021-12-25
• Completion: 2021-12-30
• First posted: 2021-11-08
• Last updated: 2023-03-15

Locations

1 site across 1 country: Turkey (Türkiye)

Regulatory

• FDA-regulated drug study