NCT04754581

Dynamic in Vivo PET Imaging and ex Vivo Biopsies From Skeletal Muscle and Adipose Tissue to Investigate the Effects of Exercise on Insulin Resistance and Mitochondrial Energetics in Type 2 Diabetes

• Status: Active Not Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 39 (actual)
• Sponsor: AdventHealth Translational Research Institute · Academic / Other
• Sex: All
• Age: 30 Years – 65 Years
• Healthy volunteers: Accepted

Summary

The overall aim of this pilot study is to investigate the effects of exercise training on skeletal muscle and adipose tissue insulin resistance in subjects with Type 2 Diabetes (T2D).

Conditions

• Type 2 Diabetes

Interventions

Type	Name	Description
OTHER	Maximal ATP Production (ATPmax)	ATPmax will be measured in order to determine in vivo mitochondrial capacity. The magnetic resonance scans to measure mitochondrial energetics will also be performed.
OTHER	Exercise Testing (VO2max)	Aerobic fitness level will be assessed while participant pedals on a stationary bicycle/walk on a treadmill. The volume of oxygen intake and carbon dioxide (CO2) production will be measured.
PROCEDURE	Muscle Biopsy	Sample muscle cells from the left leg Vastus Lateralis (thigh) muscle.
PROCEDURE	Adipose Tissue Biopsy	Sample fat tissue from the abdomen.
OTHER	Hyperinsulinemic euglycemic clamp	Measurement of insulin sensitivity.
OTHER	PET imaging	After an overnight fast, an intravenous catheter will be placed in each arm (one with arterial line for PET infusion, and the other for infusion of insulin, glucose, and 6,6 D2 glucose tracer. FDG-glucose will be injected followed by 90-minute PET scanning of the thigh.

Timeline

• Start date: 2021-03-30
• Primary completion: 2025-08-15
• Completion: 2026-12-01
• First posted: 2021-02-15
• Last updated: 2026-03-30

Locations

1 site across 1 country: United States