NCT04713436

The Effects of Antibiotic Combinations on Stem Cells

The Effects of Antibiotic Combinations on Mesenchymal Stem Cells Derived From Apical Papilla

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 3 (actual)
• Sponsor: Eskisehir Osmangazi University · Academic / Other
• Sex: All
• Age: 16 Years – 22 Years
• Healthy volunteers: Accepted

Summary

The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Detailed description

The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Conditions

• Stem Cells
• Anti-Bacterial Agents

Interventions

Type	Name	Description
DRUG	Differentiation Markers	Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Timeline

• Start date: 2014-01-01
• Primary completion: 2016-01-01
• Completion: 2017-02-01
• First posted: 2021-01-19
• Last updated: 2021-01-19

Locations

1 site across 1 country: Turkey (Türkiye)