NCT04676269

Amnion Bilayer and Stem Cell Combination Therapy on Thin Endometrium Infertile Patients

• Status: Unknown
• Phase: EARLY_Phase 1
• Study type: Interventional
• Enrollment: 40 (estimated)
• Sponsor: Indonesia University · Academic / Other
• Sex: Female
• Age: 40 Years
• Healthy volunteers: Not accepted

Summary

Severe infections or trauma to the endometrial lining causes permanent scars that disrupt the menstrual cycle and lead to conceive failure. Transplantation of biological graft seeded with stem cells is purposed to regenerate and recover the capability of the endometrial lining back into its cycles. Initially, the techniques to isolate and culture the endometrial cells and amnion epithelial stem cells were developed, then the endometrial cells form patients with thin endometrium. Tissue were obtained from hysteroscopic biopsy, weight between 100 µl, while up to 20 µl from the thin endometrium. Tissue were digested using collagenase-1 and cultured using DMEM-F12 added wit epidermal growth factor. Endometrial cells will be characterized to SSUD2, ICAM and BRCP1. Amnion epithelial stem cells (hAESC) will be isolated using collagenase-1 and hyaluronidase. Characterization towards TRA-1-60, SSEA-4, Oct 3/4, and Nanog. In the future, the cells will be co-culture on amnion bilayer, and stained using IHC against α-cadherin, estrogen receptor α, progesterone receptor. Endometrial receptivity (HOXA10, LIF (early secretory) adhesion, VEGF, osteopontin (SPP1) to indicate the pinopodes will be identified using qPCR.The SPP1, target of MIR424 expressed during the receptive phase.

Conditions

• Cell- and Tissue-Based Therapy

Interventions

Type	Name	Description
BIOLOGICAL	Amnion only	Patients endometrium will be transplanted with amnion only (without seeded cells)
BIOLOGICAL	Amnion - endometrium cells	Patients endometrium will be transplanted with amnion seeded with endometrium cells isolated from the patients themselves
BIOLOGICAL	Amnion - amnion epithelial cells	Patients endometrium will be transplanted with amnion seeded with amnion epithelial stem cells isolated from other patients' caesarean sectio amnion membrane (tested HLA-DR negative to prevent rejection)
BIOLOGICAL	Amnion-EnSC-AESC	Patients endometrium will be transplanted with amnion seeded with endometrium cells-amnion epithelial stem cells co-culture

Timeline

• Start date: 2019-12-09
• Primary completion: 2021-07-15
• Completion: 2021-12-15
• First posted: 2020-12-21
• Last updated: 2020-12-21

Locations

1 site across 1 country: Indonesia