NCT04675164

Laser Assisted Sperm Selection of Viable Immotile Testicular Sperm in Azoospermic Infertile Men

Outcome of ICSI Using Laser Assisted Sperm Selection of Viable Immotile Testicular Sperm in Azoospermic Infertile Men: a Randomized Controlled Trial

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 168 (actual)
• Sponsor: Sohag University · Academic / Other
• Sex: All
• Age: 18 Years – 35 Years
• Healthy volunteers: Not accepted

Summary

This study aims to investigate the outcome of ICSI after using LAISS for selection of viable immotile testicular sperm in azoospermic infertile men. Patients will be randomly assigned to 2 groups. In (LAISS) group, viable immotile testicular sperms will be selected before ICSI using laser assisted immotile sperm selection (study group). In (HOST) group, viable immotile testicular sperms will be selected before ICSI using hypo-osmotic swelling test (control group).

Conditions

• Azoospermia
• IVF

Interventions

Type	Name	Description
DEVICE	Laser Assisted Immotile Sperm Selection	LAISS will be performed using diode laser system at the lowest working power with a single laser shot at the lower one third of the sperm tail. If sperm tail curling occurred, the sperm is then viable and will be utilized for ICSI after immobilization in PVP.
PROCEDURE	Hypo-osmotic Swelling Test	HOST will be performed by transferring immotile sperms from incubation droplet into HOST droplet, with any sperm showing tail swelling will be identified as viable and will be utilized for ICSI after immediate transfer and immobilization.

Timeline

• Start date: 2020-10-01
• Primary completion: 2023-09-30
• Completion: 2023-09-30
• First posted: 2020-12-19
• Last updated: 2023-11-27

Locations

1 site across 1 country: Egypt