Clinical Trials Directory

Trials / Completed

CompletedNCT04547699

Cytokines Supplementation to Culture Media and ICSI Outcome

Impact of Cytokine Supplementation With Various Protein Concentrations Into Human in Vitro Culture Medium on ICSI Cycle Outcome

Status
Completed
Phase
N/A
Study type
Interventional
Enrollment
700 (actual)
Sponsor
Mansoura Integrated Fertility Center · Academic / Other
Sex
Female
Age
18 Years – 38 Years
Healthy volunteers
Accepted

Summary

A randomized clinical trial to evaluate the effect of cytokine supplementation in embryo culture medium with various protein concentrations aiming to evaluate the effects of cytokines (CYK) integration into the human in vitro culture medium with high and low human serum albumin (HSA) concentrations on live birth rates after intracytoplasmic sperm injection (ICSI).

Detailed description

Oocytes were injected, and embryos cultured and transferred in three supplemented culture media. A- A single step culture medium (SSCM; Global, Life Global)+5mg/ml (10% Vol/Vol), life global protein, without supplementation of CYK (control n=200), B- A single step culture medium (SSCM; Global, Life Global)+5mg/ml (10% Vol/Vol), life global protein, with 2ng/mL GM-CSF (granulocyte macrophage colony stimulating factor) (G5035 Sigma), 5ng/mL HB-EGF (Heparine Binding Epidermal Growth Factor )(E4643 Sigma),and 5ng/mL LIF (Lukemia Inhbitory Factor) (SRP9001 Sigma)(n=200). C- A single step culture medium (SSCM; Global, Life Global) +2mg/ml (5% Vol/Vol) life global protein, with 2ng/mL GM-CSF (G5035 Sigma), 5ng/mL HB-EGF (E4643 Sigma), and 5ng/mL LIF (SRP9001 Sigma).(n=200) Setting :- - Mansoura Integrated Fertility

Conditions

Interventions

TypeNameDescription
COMBINATION_PRODUCTA single step culture mediumA single step culture medium (SSCM; Global, Life Global)+5mg/ml life global protein, without supplementation of CYK

Timeline

Start date
2020-02-01
Primary completion
2021-07-01
Completion
2022-07-01
First posted
2020-09-14
Last updated
2023-06-18

Locations

2 sites across 1 country: Egypt

Source: ClinicalTrials.gov record NCT04547699. Inclusion in this directory is not an endorsement.