NCT04535648

Detection of Enterovirus Genotypes by CRISPR Technology

Rapid Identification and Clinical Transformation of Various Enterovirus Genotypes Based on CRISPR Technology

• Status: Withdrawn
• Phase: —
• Study type: Observational
• Enrollment: 0 (actual)
• Sponsor: Children's Hospital of Fudan University · Academic / Other
• Sex: All
• Age: 1 Day – 18 Years
• Healthy volunteers: Not accepted

Summary

CRISPR-enterovirus detection system was constructed in this study for detection variety genotypes of enterovirus rapidly in children suspected or diagnosed as enterovirus infection.

Detailed description

To construct CRISPR-enterovirus detection system, we screened the targets, primers and CrRNA and evaluated the capability of this detecting system. Samples of feces, blood and cerebrospinal fluid (the remainder of routine testing samples) from 500 children with suspected or confirmed enterovirus infection were examined using the CRISPR technique. To evaluate the CRISPR-enterovirus detection system, real-time PCR and traditional PCR as comparison method was implemented. The method of real-time PCR and traditional PCR were used to detect enterovirus and analyze the genotypes of enterovirus, respectively. According to the results of CRISPR-enterovirus detection system and the comparison method, the positive coincidence rate, negative coincidence rate and consistency of the two methods were calculated. Furthermore, the sensitivity and specificity of the CRISPR-enterovirus detection system for detection of enterovirus were calculated.

Conditions

• Enterovirus Infections

Interventions

Type	Name	Description
OTHER	Non-invasive detection method: CRISPR techonology	This study was a laboratory diagnostic study without any intervention.

Timeline

• Start date: 2023-04-01
• Primary completion: 2024-08-01
• Completion: 2024-09-01
• First posted: 2020-09-02
• Last updated: 2023-06-09