NCT04485806

Culture Media Microdroplet Geometry With Different Oil Overlay and Its Effect on Embryonic Development.

The Effect of Culture Media Microdroplet Geometry and Different Oil Overlay on Pre Implantation Embryonic Development in Dry Incubators: Prospective Randomized Study.

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 100 (actual)
• Sponsor: Ganin Fertility Center · Academic / Other
• Sex: Female
• Age: 18 Years – 37 Years
• Healthy volunteers: Not accepted

Summary

A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.

Detailed description

Purpose: to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators Method: We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions. statistical analysis done using SPSS version 23

Conditions

• Embryonic Development
• Infertility

Interventions

Type	Name	Description
OTHER	GPS/LM	Media microdroplet geometry (3D dishes) in combination with light mineral oil overlay
OTHER	SPL/LM	Media microdroplet geometry (2D dishes) in combination with light mineral oil overlay
OTHER	GPS/PO	Media microdroplet geometry (3D dishes) in combination with Paraffin oil overlay
OTHER	SPL/PO	Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay

Timeline

• Start date: 2019-08-01
• Primary completion: 2020-01-01
• Completion: 2020-01-10
• First posted: 2020-07-24
• Last updated: 2020-07-24

Locations

1 site across 1 country: Egypt