NCT04361448

Performance of 3 Sampling Methods for the Detection of SARS-CoV-2 (COVID-19) With Real-time Reverse Transcriptase PCR

Comparison of the Performance of 3 Sampling Methods for the Detection of SARS-CoV-2 (COVID-19) With Real-time Reverse Transcriptase PCR

• Status: Terminated
• Phase: N/A
• Study type: Interventional
• Enrollment: 30 (actual)
• Sponsor: Jessa Hospital · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Accepted

Summary

The performance of 3 different sampling methods (2 nasopharyngeal swabs, 1 oropharyngeal swab) for the detection of SARS-CoV-2 with real-time reverse transcriptase polymerase chain reaction will be compared.

Detailed description

Thirty volunteers will be recruited from health care workers suspicious for Covid-19, who consult the ocupational physician for Covid-19 testing. For each volunteer, 3 swabs will be taken: * Flocked eSwab (Copan, Italy) of the oropharynx * Flocked nasopharyngeal eSwab (Copan, Italy) of the nasopharynx * Dry flocked nasopharyngeal Swab, placed in lysis buffer Each swab will be tested for the presence of SARS-CoV-2 with an in-house reverse-transcriptase real-time PCR (Egene). Performance of each method will be calculated.

Conditions

• Covid-19
• SARS-CoV-2
• Diagnosis
• Polymerase Chain Reaction

Interventions

Type	Name	Description
OTHER	samling of oropharynx and nasopharynx	Three different swabs will be taken of each participant: 1 oropharyngeal swab and 2 nasopharyngeal swabs

Timeline

• Start date: 2020-04-20
• Primary completion: 2020-06-30
• Completion: 2020-06-30
• First posted: 2020-04-24
• Last updated: 2021-02-15

Locations

1 site across 1 country: Belgium