NCT04277182

Effect of Propolis Application on Burn Healing

Effect of Topical Turkish Propolis Application on Burn Healing in Experimental Burn Model

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 36 (actual)
• Sponsor: Izmir Katip Celebi University · Academic / Other
• Sex: All
• Age: 8 Weeks – 12 Weeks
• Healthy volunteers: Accepted

Summary

This experimental study will be carried out at Ege University Laboratory Animals Application and Research Center to identify the efficacy of 10% propolis and 15% propolis on wound healing in a second-degree superficial burn wound. In the study, 36 adult (8-12 week old) Wistar-Albino rats with a weight of 200-300 grams will be used in each group. Each group will include 6 rats. Stratified randomization will be used to randomize rats according to their gender and weight. After randomization, a burnt area will be created on the back of the rats and dressings will be made every day. Following the creation of the burn, biopsy samples will be taken on the 3rd, 7th, 14th and 21st days and at the light microscopic level; bulla, erythema, oedema, burn depth, inflammatory cell infiltration, necrosis, ulceration, angiogenesis, neovascularization, fibroblast proliferation, collagenization, epithelization, fibrosis, the number of hair follicles and damage to the skin attachments. In the immunohistochemical examination of biopsy samples, samples will be taken for the evaluation of superoxide dismutase, malondialdehyde, myeloperoxidase in the evaluation of the tumour necrosis factor-alpha, interleukin-1β and oxidative stress in the EBB period in order to evaluate the acute phase reactants. Fungal and gram staining will be done in order to determine the pathogen reproduction in biopsy samples taken on the 3rd and 7th day. Masson trichrome in order to detect fibrosis in biopsy samples taken on the 14th and 21st day; Hydroxyproline examination will be examined in order to evaluate collagen formation.

Conditions

• Burns
• Burn Wound

Interventions

Type	Name	Description
BIOLOGICAL	burn wound dressing	1. st. group: It will be considered as a control group and rats will not be treated. 2. nd. group: Vehicle group, Propolis vehicle will be applied topically to rats for 21 days. 3. rd group: Silver Sulfadiazine group, 1% Silver Sulfadiazine will be applied topically to rats for 21 days. 4. th. group: Nitrafurozon group, 0.2% Nitrafurozon will be applied topically for 21 days. 5. th. group: 10% Propolis group, 10% Propolis will be applied topically for 21 days. 6. th. group: 15% Propolis group, 15% Propolis will be applied topically for 21 days.

Timeline

• Start date: 2020-09-01
• Primary completion: 2020-09-28
• Completion: 2020-09-28
• First posted: 2020-02-20
• Last updated: 2022-08-03

Locations

1 site across 1 country: Turkey (Türkiye)