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CompletedNCT04266587

ACTH Stability on Whole Blood

Adrenocorticotropic Hormone Stability on Whole Blood

Status
Completed
Phase
N/A
Study type
Interventional
Enrollment
10 (actual)
Sponsor
University Hospital, Rouen · Academic / Other
Sex
All
Age
18 Years – 65 Years
Healthy volunteers
Accepted

Summary

ACTH is a peptide secreted by pituitary gland and plays an important role in regulating cortisol secretion. ACTH is determined in plasma by immunoassays using specific antibodies. Its determination is difficult because of instability in whole blood. Several factors which influence ACTH stability in blood before analysis have been identified: temperature, hemolysis, time to centrifugation and presence of protease inhibitors. Published results on ACTH whole blood stability seem contradictory. The objective of this study is to evaluate the effect of aprotinin in 10 healthy volunteers. ACTH measurements will be performed on cobas e602 (Roche Diagnostics, Mannheim, Germany).

Conditions

Interventions

TypeNameDescription
BIOLOGICALBlood samplingblood sampling is done on healthy volunteers

Timeline

Start date
2021-11-18
Primary completion
2021-11-18
Completion
2021-11-18
First posted
2020-02-12
Last updated
2026-02-10

Locations

1 site across 1 country: France

Source: ClinicalTrials.gov record NCT04266587. Inclusion in this directory is not an endorsement.