NCT04137029

Methacholine, Long-acting M-cholinolytic and beta2-agonist on the Activity of Beta-receptors in Healthy Volunteers

Effect of Methacholine, Long-acting M-cholinolytic and beta2-agonist on the Binding Activity of Beta-receptors in Healthy Volunteers

• Status: Unknown
• Phase: N/A
• Study type: Interventional
• Enrollment: 20 (estimated)
• Sponsor: Federal State Budgetary Institution, Pulmonology Scientific Research Institute · Other Government
• Sex: All
• Age: 18 Years – 60 Years
• Healthy volunteers: Accepted

Summary

Interventional open-labeled study in parallel groups is aimed to evaluate changes of beta receptors in healthy volunteers under the influence of inhaled metacholine, long-acting anticholinergics and beta-agonists using modified radioligand method.

Detailed description

Long-acting beta2-agonists and anticholinergics are main drugs in therapy of chronic obstructive pulmonary disease . Beta-agonists exert their bronchodilatory effects via β2 adrenoceptors located on airway smooth muscle cells. Anticholinergics block mainly M3-cholinoreceptors located in the airways, thus inhibiting bronchoconstriction. Metacholine blocks M2-cholinoreceptors thus inducing bronchoconstriction. It is known, that muscarine and beta-receptors interact with each other, but the detailed mechanism is unknown. For evaluating the binding activity of beta-receptors after inhalation of these drugs the modified radioligand method was used.

Conditions

• Health, Subjective
• Hyperreactivity, Bronchial

Interventions

Type	Name	Description
DRUG	Formoterol	The participants will receive inhalation of formoterol 12 micrograms
DRUG	Tiotropium Bromide	The participants will receive inhalation of tiotropium bromide 18 mkg once
DIAGNOSTIC_TEST	metacholine test	The participants will receive inhalation of metacholine once

Timeline

• Start date: 2019-04-24
• Primary completion: 2020-07-01
• Completion: 2020-10-01
• First posted: 2019-10-23
• Last updated: 2019-10-23

Locations

1 site across 1 country: Russia