NCT04122794

The Role of Unsteady Wall Shears Stress Stimuli on Human Umbilical Vein Endothelial Cells Harvested From Umbilical Cords

The Role of Unsteady Wall Shears Stress Stimuli on Human Umbilical Vein Endothelial Cells (HUVECs) Harvested From Umbilical Cords

• Status: Terminated
• Phase: —
• Study type: Observational
• Enrollment: 13 (actual)
• Sponsor: University Hospital of Limerick · Academic / Other
• Sex: Female
• Age: 18 Years – 40 Years
• Healthy volunteers: —

Summary

The aim of this study is to obtain and test endothelial cells (EC's) from umbilical cords to examine specific properties of these cells which make up the inner lining of blood vessels.

Detailed description

Mechanical testing will be performed on the Ec's to see how the cells reacts and ultimately how the blood vessel is affected. A central role in the mechanism that defines the nature of vessel remodelling is played by endothelial cells. EC's are extremely sensitive to the frictional forces exerted on a unit of the endothelial area by blood flow. This mechanical stimulus is known as wall shear stress (WSS). WSS experienced by EC's in-vivo is unsteady due to the unsteady nature of blood flow produced during the cardiac cycle and due to the complex nature of the vascular tree. The extent to which this applies to venous cells and tissue remains unknown and it is the focus of this study.

Conditions

• Vascular Diseases

Timeline

• Start date: 2019-11-20
• Primary completion: 2023-03-22
• Completion: 2024-03-22
• First posted: 2019-10-10
• Last updated: 2024-04-12

Locations

1 site across 1 country: Ireland