NCT03935373

DNA Exome Sequencing TCM for Sjögren's Syndrome

Using the DNA Exome Sequencing Tools to Explore the Association Among Gene Sequence, TCM Pattern, TCM Tongue Diagnosis and TCM Pulse Diagnosis for Sjögren's Syndrome

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 40 (estimated)
• Sponsor: Taipei Veterans General Hospital, Taiwan · Other Government
• Sex: All
• Age: 20 Years – 75 Years
• Healthy volunteers: Accepted

Summary

To explore the association among gene, TCM pattern, TCM tongue diagnosis and TCM pulse diagnosis with the DNA Exome sequencing tools for Sjögren's syndrome

Detailed description

Objective: To explore the association among gene, TCM pattern, TCM tongue diagnosis and TCM pulse diagnosis with the DNA Exome sequencing tools for Sjögren's syndrome Method: This study wants to establish the reference value of DNA gene variants of healthy control for comparing with the Sjögren's syndrome subjects. And the investigators want to use the DNA Exome sequencing tools, proteomics and oral microbiome to explore the association of gene expression between at least 30 Sjögren's syndrome subjects and at least 10 healthy control subjects. Finally, the investigators also analyze the association among gene expression, proteomics and oral microbiome, TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, and heart rate variability (HRV) for this purpose. Expected Results: 1. To evaluate the difference among TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, and HRV for the Sjögren's syndrome and healthy control. 2. To evaluate the immunity-related gene expression for the Sjögren's syndrome and healthy control. 3. To evaluate the proteomics for the Sjögren's syndrome and healthy control. 4. To evaluate the oral microbiome for the Sjögren's syndrome and healthy control. 5. To evaluate the association among the immunity-related gene expression, SSA, SSB and RF. Keyword: Sjögren's syndrome, Immune regulation, Gene modulation, TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, proteomics, oral microbiome

Conditions

• Sjögren's Syndrome

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	DNA Exome sequencing	The DNA of Sjögren's syndrome patients and health subjects will take to do the DNA Exome sequencing.
DIAGNOSTIC_TEST	Oral microbiome	The saliva of Sjögren's syndrome patients and health subjects will take to do the oral microbiome.
DIAGNOSTIC_TEST	Proteomics	The protein of Sjögren's syndrome patients and health subjects will take to do the proteomics.
DIAGNOSTIC_TEST	RNA sequencing	The RNA of Sjögren's syndrome patients and health subjects will store to do the RNA sequencing.
DIAGNOSTIC_TEST	Cytokine	The serum of Sjögren's syndrome patients and health subjects will store to do the cytokine detection in the future.
DIAGNOSTIC_TEST	Traditional Chinese Medicine pattern	Sjögren's syndrome patients and health subjects will take the Traditional Chinese Medicine pattern.
DIAGNOSTIC_TEST	Traditional Chinese Medicine tongue diagnosis	Sjögren's syndrome patients and health subjects will take the Traditional Chinese Medicine tongue diagnosis.
DIAGNOSTIC_TEST	Traditional Chinese Medicine pulse diagnosis	Sjögren's syndrome patients and health subjects will take the Traditional Chinese Medicine pulse diagnosis.
DIAGNOSTIC_TEST	Heart rate variability	Sjögren's syndrome patients and health subjects will take the heart rate variability.
DIAGNOSTIC_TEST	Schirmer's test	Sjögren's syndrome patients will take the Schirmer's test.
DIAGNOSTIC_TEST	Blood function test, liver function test, kidney function test, and immune function test	Sjögren's syndrome patients and health subjects will take the blood function test (WBC, RBC, Hb, HCT, Platelet, MCV, MCH, and MCHC), liver function test (AST and ALT), kidney function test (BUN and Cre), and immune function test (RF, Anti-SSA, Anti-SSB Ab, ESR, and CRP)

Timeline

• Start date: 2019-04-01
• Primary completion: 2019-05-15
• Completion: 2019-12-31
• First posted: 2019-05-02
• Last updated: 2019-05-06

Locations

1 site across 1 country: Taiwan