NCT03879187

Molecular and Hormonal Responses to Diet-Induced Insulin Resistance

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 15 (actual)
• Sponsor: Loughborough University · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.

Detailed description

Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.

Conditions

• Healthy

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	High-fat, high-energy diet	Participants consumed a diet that was high in fat and high in energy

Timeline

• Start date: 2015-03-02
• Primary completion: 2018-05-22
• Completion: 2018-05-22
• First posted: 2019-03-18
• Last updated: 2019-03-18