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Trials / Completed

CompletedNCT03857984

Hemodialysis and Erythrocyte Fatty-Acid Status: a Lipidomics Study

Status
Completed
Phase
N/A
Study type
Interventional
Enrollment
30 (actual)
Sponsor
Benjamin Gollasch · Academic / Other
Sex
All
Age
18 Years – 75 Years
Healthy volunteers
Accepted

Summary

Hypotension with potentially serious consequences for organ perfusion is a common complication in extracorporeal procedures such as hemodialysis / hemofiltration. The exact reasons for this are still insufficiently clarified. Probably periinterventional vasorelaxant released substances play a crucial role in these procedures. These substances could be due to contact of blood cells with the Membrane in the dialyzer / hemofilter arise. In this project the hypothesis will be checked, if EETs / DHETs are released by Erythrocytes during hemodialysis and thus act as potential candidate products for the result of hypotonic phases in the Dialysis. We will determine differences in RBC fatty acids profiling in hemodialysis patients before and after dialysis intervention. Furthermore a matched control group of healthy individuals will be profiled. RBC fatty acids profiling will be achieved by using targeted HPLC-MS mass spectrometry. It is believed that during hemodialysis / HLM / CVVH there is an increase in EETs / DHETs in the serum and in the erythrocytes. It is believed that shear forces play an important role in the release of erythrocyte EETs / DHETs.

Detailed description

Differences in Erythrocyte Fatty-Acid Status Profiling in: * before and after hemodialysis intervention * in hemodialysis patients and healthy individuals Venous blood will be collected from each subject in the sitting position

Conditions

Interventions

TypeNameDescription
OTHERBlood samplingblood sample before and after a single HD

Timeline

Start date
2018-05-01
Primary completion
2022-01-01
Completion
2022-02-01
First posted
2019-02-28
Last updated
2022-03-10

Locations

1 site across 1 country: Germany

Source: ClinicalTrials.gov record NCT03857984. Inclusion in this directory is not an endorsement.

Hemodialysis and Erythrocyte Fatty-Acid Status: a Lipidomics Study (NCT03857984) · Clinical Trials Directory