NCT03842371

Metabolism of Branched-chain Amino Acids in Monocyte/Macrophage During Sepsis

Metabolism of Branched-chain Amino Acids in Monocyte and Macrophage During Sepsis

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 90 (estimated)
• Sponsor: West China Hospital · Academic / Other
• Sex: All
• Age: 18 Years – 70 Years
• Healthy volunteers: Accepted

Summary

The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Detailed description

In this study, the investigators will collect the peripheral blood of 1,3,7,14 day after diagnose of sepsis. Then, the concentration of branched-chain amino acids(BCAAs) in monocytes and plasma, and the expression of BCAAs metabolic enzymes in monocytes will be determined. Additionally, the inflammatory cytokines, e.g., Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), Interleukin-10 (IL-10）, the chemokines macrophage inflammatory proteins 1α and 1β (MIP-1α and 1β) in the peripheral blood and the phagocytic function of monocytes will also be detected. The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Conditions

• Sepsis Syndrome

Interventions

Type	Name	Description
OTHER	Metabolic level of BCAAs in monocytes and macrophages	Metabolic level of BCAAs in monocytes and macrophages

Timeline

• Start date: 2019-02-11
• Primary completion: 2021-12-30
• Completion: 2022-02-28
• First posted: 2019-02-15
• Last updated: 2019-02-15