NCT03763487

The Aim of This Project is to Verify Whether a Depletion of B Cell Memory Subpopulation ("Marginal-zone-like") CD19 + IgD + CD27 + and CD19 + IgM + CD27 + is a Useful Indicator of Hyposplenism in Patients With Celiac Disease

Marginal Zone Similar to B Lymphocytes in Peripheral Blood in Patients With Celiac Disease

• Status: Unknown
• Phase: N/A
• Study type: Interventional
• Enrollment: 50 (estimated)
• Sponsor: University Hospital Hradec Kralove · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

This project is expected to confirm the hypothesis that hyposplenism in patients with celiac disease is not conditioned by a selective memory deficiency of B lymphocytes. Other objectives of project are: * determination of gliadin 33-mer in faecal and urine as indicators patient´s adherence to gluten-free diet * determination of citrulline in plasma as an indicator of the overall functional capacity enterocytes

Detailed description

Ultrasound examination is focused to spleen size (spleen volumetry). Methodology of laboratory examinations is based on venous blood sampling for the determination of memory B-like marginal zone by flow cytometry; gliadin 33-mer (ELISA) determination in faecal and urine sample; blood plasma extraction for citrulline (high performance liquid chromatography, HPLC).

Conditions

• Celiac Disease

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	venous blood sampling	Venous blood samples to determine memory B lymphocytes.
PROCEDURE	spleen volumetry	Ultrasound examination of spleen to scaled the size.

Timeline

• Start date: 2018-06-01
• Primary completion: 2018-12-31
• Completion: 2018-12-31
• First posted: 2018-12-04
• Last updated: 2018-12-04

Locations

1 site across 1 country: Czechia