Trials / Completed
CompletedNCT03728192
In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines
Inhibition and Cytotoxic Effects of Mangosteen on Cell Lines
- Status
- Completed
- Phase
- N/A
- Study type
- Interventional
- Enrollment
- 2 (actual)
- Sponsor
- Meenakshi Ammal Dental College and Hospital · Academic / Other
- Sex
- All
- Age
- —
- Healthy volunteers
- Not accepted
Summary
The present study is an effort to investigate the hypothesis that in-vitro vitality and antiapoptotic effect of alcoholic crude extract of mangosteen on Oral cancer( H357) cell lines and Cevical cancer (HeLa) cell lines.
Detailed description
The oral and cervical cells were investigated by MTT (3-4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, DNA fragmentation detection by TUNEL (Terminal deoxynucleotidyl transferase-mediated d-UTP Nick End Labeling) assay and Apototic assay using Annexin V/FITC Kit.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| OTHER | mangosteen extract | For Mangosteen group :DNA fragmentation assay is used to identify apoptosis.1×106 cells were harvested and treated with mangosteen pericarp extract for 48 h For Camptothecin group : Cells were also treated with standard anticancer drug Camptothecin to act as a positive control. |
Timeline
- Start date
- 2016-03-07
- Primary completion
- 2016-05-11
- Completion
- 2018-07-13
- First posted
- 2018-11-02
- Last updated
- 2018-11-02
Source: ClinicalTrials.gov record NCT03728192. Inclusion in this directory is not an endorsement.