NCT03697928

Markers of Carnitine Acetyltransferase (CrAT) Protein Activity and Carnitine Availability

Searching for Non-invasive Magnetic Resonance-based Markers for CrAT Protein Activity and Carnitine Availability in Skeletal Muscle

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 13 (actual)
• Sponsor: Maastricht University · Academic / Other
• Sex: Male
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

This study aims to measure skeletal muscle metabolism in vivo during exercise using the non-invasive Magnetic Resonance Spectroscopy (MRS) technique. Specifically, phosphocreatine (PCr) kinetic and Acetylcarnitine levels will be determined at resting, during exercise and during the recovery post exercise. The target population is adult healthy men, with a wide range of maximal physical capacity.

Detailed description

Skeletal muscle mitochondrial activation during the transition from resting metabolism to exercise may modulated by CrAT activity. Furthermore, dynamic changes in acetylcarnitine during the transition from exercise to resting metabolism (acetylcarnitine off-kinetic) may also reflect the action of CrAT protein. To date, the only possibility to get information on CrAT activity is by taking a muscle biopsy and perform enzyme activity assays ex vivo. By dynamic MRS scanning, it might be possible to deduce information that reflects CrAT activity, thereby making the biopsy sampling unnecessary.

Conditions

• Metabolism and Nutrition Disorder
• Metabolic Disturbance

Interventions

Type	Name	Description
OTHER	Exercise metabolism	Participants will perform a series of exercises inside and outside the MRS/MRI scanner.

Timeline

• Start date: 2019-03-12
• Primary completion: 2020-02-01
• Completion: 2020-02-01
• First posted: 2018-10-05
• Last updated: 2020-10-19

Locations

1 site across 1 country: Netherlands