Trials / Completed
CompletedNCT03660904
A Comparison Between Home-made Vitrification & Thawing Media and a Commercially Available Brand on Blastocyst
A Comparison Between Home-made Vitrification & Thawing Media and a Commercially Available Brand on Blastocyst Survival, Expansion and Implantation
- Status
- Completed
- Phase
- Phase 2 / Phase 3
- Study type
- Interventional
- Enrollment
- 74 (actual)
- Sponsor
- Benha University · Academic / Other
- Sex
- Female
- Age
- 22 Years – 37 Years
- Healthy volunteers
- Not accepted
Summary
This study was conducted to evaluate and compare cryo-survival, re-expansion, and implantation rates of Day 5/6 human blastocysts that were vitrified with home-made media (HM) according to (Shady grove protocol) and warmed with gradual Thawing HM according to (Kuwayama protocol)with Global Fast Freeze/Thaw Kits (LifeGlobal, Canada).
Detailed description
Group 1 blastocysts were vitrified with the Vit HM according to shady grove protocol using open system on cryotopvit. device and thawed with gradual thawing technique using HM according to Kuwayama protocol. Group 2 blastocysts were cryopreserved with the Global Fast Freeze Kitusing open system on cryotopvit. device and thawed using Gobal fast thaw kit. Blastocysts were subsequently cultured for 6-8 h, assessed for survival and expansion, then transferred and the percentages of Positive hCG, clinical pregnancy, implantation, multiple pregnancy and abortion were recorded.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| BIOLOGICAL | home made vitrification & thawing kit | home made vitrification was made and prepared for vitrification of blastocysts then thawing with a prepared thawed media too |
| BIOLOGICAL | commercially made vitrification & thawing kit | commercially available vitrification \& thawing kit were made and prepared for vitrification of blastocysts then thawing with a prepared thawed media too |
Timeline
- Start date
- 2015-10-01
- Primary completion
- 2017-10-01
- Completion
- 2018-06-15
- First posted
- 2018-09-07
- Last updated
- 2018-09-07
Source: ClinicalTrials.gov record NCT03660904. Inclusion in this directory is not an endorsement.