Trials / Completed
CompletedNCT03623659
pArtiaL zonA pelluciDa Removal by assisteD hatchINg of Blastocysts
Does Partial Zona Pellucida Removal From Vitrified-warmed Human Blastocysts Improve Delivery Rate in IVF? A Multicentric RCT on Laser Assisted Hatching
- Status
- Completed
- Phase
- N/A
- Study type
- Interventional
- Enrollment
- 700 (actual)
- Sponsor
- IRCCS San Raffaele · Academic / Other
- Sex
- Female
- Age
- 18 Years – 40 Years
- Healthy volunteers
- Not accepted
Summary
The aim of study is to assess the possible impact of assisted hatching on delivery rate after transfer of vitrified-warmed human blastocysts.
Detailed description
Zona pellucida (ZP) manipulation, termed "assisted hatching" (AH), has been introduced in order to favor embryo hatching and ultimately improve assisted reproductive technology success but with poor proofs of safety and biological plausibility. Vitrifying and warming of blastocysts may impair the successful hatching process of the embryo out of its ZP and its following implantation into the uterus. Theoretically, AH may facilitate the hatching process and subsequently increase implantation rates. In this prospective randomized controlled trial (RCT), the hypothesis is to test whether the application of a partial AH to vitrified/warmed blastocysts might affect patients' delivery rate. Patients with vitrified blastocysts will be randomized at the time of blastocyst warming to a study group (with AH) or a control group (without AH). AH will be performed at the expanded blastocyst stage using a laser technique and a laser opening will be initiated at the 1 o'clock position. Consecutive laser shots will be applied to reach the 5 o'clock position of the blastocyst. The blastocysts will then be cultured at least 2 h and subsequently transferred into the patient's uterus.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| PROCEDURE | Laser assisted hatching | After warming, blastocysts are subjected to laser assisted hatching (LAH) following the standard procedure.The LAH procedure lasts one minute per blastocyst. |
Timeline
- Start date
- 2018-09-05
- Primary completion
- 2023-01-20
- Completion
- 2023-01-20
- First posted
- 2018-08-09
- Last updated
- 2023-01-23
Locations
2 sites across 1 country: Italy
Source: ClinicalTrials.gov record NCT03623659. Inclusion in this directory is not an endorsement.