NCT03583281

Effects of Dietary Fatty Acids on Octadecanoid Production and Biological Actions in Obesity-induced Inflammation

Effects of Dietary Fatty Acids on Octadecanoid Production and Biological Actions in Obesity-induced Inflammation: Implications for Dietary Requirements

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 24 (actual)
• Sponsor: University of Manitoba · Academic / Other
• Sex: Female
• Age: 20 Years – 55 Years
• Healthy volunteers: Not accepted

Summary

This is a single site, double-blind, randomized, crossover study designed to compare the effects of dietary supplementation with flax oil rich in alpha-linolenic acid (ALA) and fish oil rich in docosahexaenoic acid (DHA) on oxylipin profiles over time (0 to 4 weeks) and among obese females varying in their inflammatory state. Additional assessments will include plasma fatty acid composition, adipokines, markers of adipocyte dysfunction and inflammation; immune cell/monocyte fatty acid composition, and functional properties including their metabolism and oxylipin production; and vascular function.

Detailed description

A single site, double-blind, randomized, cross-over study designed to compare the effects of flax oil and fish oil supplementation on the oxylipin profile in females with obesity (n=24). Eligible participants will complete two supplementation phases (flax oil and fish oil rich in DHA) and will be asked to attend 3 in-person clinic visits (0, 3, and 28 days) for blood and urine collection during each phase. In addition to oxylipin profiles, assessments will include plasma fatty acid composition, adipokines, markers of adipocyte dysfunction and inflammation; immune cell/monocyte fatty acid composition, and functional properties including their metabolism and oxylipin production; and vascular function. If participants sign an optional consent form, vaginal fluid samples will be obtained at 0 and 28 days of each Supplementation Phase and analyzed for oxylipin and immune cell profile.

Conditions

• Obesity
• Inflammation

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Capsules containing flax oil	Capsules containing flax oil to provide 4 grams ALA per day for 4 weeks
DIETARY_SUPPLEMENT	Capsules containing DHA-enriched fish oil	Capsules containing fish oil to provide 4 grams DHA per day and 0.8 grams EPA per day for 4 weeks

Timeline

• Start date: 2018-06-01
• Primary completion: 2020-04-30
• Completion: 2021-04-30
• First posted: 2018-07-11
• Last updated: 2021-07-02

Locations

1 site across 1 country: Canada