Trials / Completed
CompletedNCT03549533
Impact of Aluminum on Sperm DNA Quality
Impact of Aluminum on Sperm DeoxyriboNucleic Acid (DNA) Quality
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 80 (actual)
- Sponsor
- Centre Hospitalier Universitaire de Saint Etienne · Academic / Other
- Sex
- Male
- Age
- 18 Years
- Healthy volunteers
- Not accepted
Summary
Between 1950 and 2013, aluminum production was multiplied by thirty in the world. Today, men's exposure to aluminum, including food products, cosmetics, air and water contamination, and a number of drugs (vaccine, gastric bandages, etc.) has never been so high and should continue to increase. At the same time, we are witnessing a decline in male fertility in Western countries. In this context, several teams, including ours, have studied the impact of aluminum on the fertility of men. These studies have shown that aluminum accumulates in semen and especially in sperm near its DNA. Aluminum has already shown that it is capable of damaging the DNA of various cells, especially to increase DNA fragmentation. We therefore hypothesize that aluminum could lead to increased sperm DNA fragmentation. This would result in a decrease of men fertility and in higher risk of miscarriage.
Detailed description
In order to confirm this hypothesis, this study aims to dose aluminum in the spermatozoa of 80 patients who perform artificial insemination and correlate this result to their sperm DNA fragmentation. Patients will be recruited from the reproductive biology unit of Saint-Etienne University Hospital. Aluminum assays will be carried out using an Atomic Absorption Spectrophotometry technique by Prof. Exley at Keele University in Great Britain, a world expert in aluminum toxicity. Measurements of sperm DNA damage will be carried out using flow cytometry by our research team (SAINBIOSE INSERM U1059) at the Medecine Faculty of Saint-Etienne.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| OTHER | samples of sperm | As in the usual practice during artificial insemination a samples of sperm will be collected and analyzed. |
Timeline
- Start date
- 2018-06-13
- Primary completion
- 2021-09-07
- Completion
- 2021-09-22
- First posted
- 2018-06-08
- Last updated
- 2022-03-11
Locations
1 site across 1 country: France
Source: ClinicalTrials.gov record NCT03549533. Inclusion in this directory is not an endorsement.