Trials / Unknown
UnknownNCT03456739
The Microbiome Variability and Antibiotic Resistance of Chronic Suppurative Otitis
The Microbiome Variability and Antibiotic Resistance of Chronic Suppurative Otitis Media in Pediatric Patients in Northern Israel
- Status
- Unknown
- Phase
- —
- Study type
- Observational
- Enrollment
- 240 (estimated)
- Sponsor
- Ziv Hospital · Other Government
- Sex
- All
- Age
- 1 Year – 9 Years
- Healthy volunteers
- Not accepted
Summary
ear swabs will be collected from 120 patients with established CSOM at the ear-nose-throat (ENT) outpatient clinic at the Ziv medical center, and 120 swabs will be taken from children with Otitis Media with Effusion (OME) undergoing elective tympanostomy tube insertion, serving as a control group. Ear swabs will be frozen at -80C until analysis.DNA will be purified from ear swabs, and amplified by PCR so that a barcoded 16S rRNA library from each subject will be generated. Using correlation analysis, we will compare between microbial compositions in chronic otorrhea patients vs. control groups.An additional ear swab will be collected from patients and cultured to test antibiotic resistance.
Conditions
Timeline
- Start date
- 2018-04-20
- Primary completion
- 2019-04-20
- Completion
- 2020-02-20
- First posted
- 2018-03-07
- Last updated
- 2018-03-07
Source: ClinicalTrials.gov record NCT03456739. Inclusion in this directory is not an endorsement.