NCT03413046

The Effect of Chemotherapy on Regulatory T Cells

The Effect of Chemotherapy on Regulatory T Cells in Children With Acute Lymphoblastic Leukemia

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 60 (estimated)
• Sponsor: Ankara Children's Health and Diseases Hematology and Oncology Training and Research Hospital · Other Government
• Sex: All
• Age: 1 Year – 18 Years
• Healthy volunteers: Accepted

Summary

The investigators aimed to study the effect of chemotherapy on regulatory T cells (Tregs) in children with precursor B (PreB) acute lymphoblastic leukemia (ALL) at 15 and 33 days of malignity directed therapy. The investigators will analyze peripheral and bone marrow blood samples of the participants, obtained at diagnosis, 15 and 33 days of treatment, about Treg / non Treg cells and interleukin (IL)-2, IL-6, IL-10, and transforming growth factor (TGF) beta levels. By this means, investigators will search response to chemotherapy. Also, investigators will analyze correlation between Treg population-Treg related cytokines with demographic, clinical and laboratory findings of the participants with ALL at these certain time points. Additionally, the investigators will compare Treg population and minimal residual disease at 15-33 days of malignity directed therapy. Also investigators will compare these Treg-Treg related cytokines obtained from the participants with ALL and a healthy children control group whom are voluntary donors for bone marrow transplantation.

Conditions

• Pre B ALL
• Regulatory T Cell

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Peripheral and bone marrow sampling	We obtained blood from peripheral and bone marrow sampling. Then we analyze Treg population and Treg related cytokines with flow cytometry and ELISA methods.

Timeline

• Start date: 2018-01-01
• Primary completion: 2019-01-01
• Completion: 2019-05-01
• First posted: 2018-01-29
• Last updated: 2018-01-29