NCT03404557

Proteomic Analysis of Crohn's Disease Macrophages in Response or Not to AIEC

Proteomic Analysis of Crohn's Disease Macrophages in Response or Not to Adherent-Invasive Escherichia Coli

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 88 (actual)
• Sponsor: University Hospital, Clermont-Ferrand · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

The M2iSH laboratory showed with two previous clinical trials that Crohn's Disease (CD) macrophages present i) a defect to control Adherent-Invasive Escherichia coli (AIEC) infection related to polymorphisms associated with CD; ii) a CD - specific cytokine secretion profile after an AIEC infection and intestinal inflammation dependent; iii) a modification of the response of CD macrophages at a basal state and after the AIEC infection. These results consolidate the hypothesis of a defect specific to CD macrophages. That's why, the primary purpose of this study is to realize a proteomic analysis of macrophages of CD patients infected or not with AIEC and to compare them to Ulcerative Colitis (UC) patients and healthy volunteers.

Detailed description

The macrophages characterization will be realized at a basal state and in response or not to AIEC. Investigator aimed to 1) better understand the differences between macrophages of CD patients, UC patients and healthy volunteers but also the impact of AIEC infection on these macrophages to provide key informations to detect and characterize the defect of these macrophages; 2) highlight one or several protein in CD macrophages that could represent, in a long term, a potential therapeutic target of CD.

Conditions

• Crohn's Disease
• Ulcerative Colitis

Interventions

Type	Name	Description
OTHER	Proteomic analysis	Proteomic analysis of Crohn's disease macrophages in response or not to Adherent-Invasive Escherichia coli

Timeline

• Start date: 2018-01-18
• Primary completion: 2019-01-18
• Completion: 2019-01-18
• First posted: 2018-01-19
• Last updated: 2019-02-04

Locations

1 site across 1 country: France