NCT03315325

Age-dependent Changes in the Responsiveness of Hypothalamic Pituitary Gonadal Axis in Men

Age-dependent Changes in the Responsiveness of Hypothalamic Pituitary Gonadal Axis to Kisspeptin-10 Administration in Men

• Status: Completed
• Phase: Phase 3
• Study type: Interventional
• Enrollment: 15 (actual)
• Sponsor: Quaid-e-Azam University · Academic / Other
• Sex: Male
• Age: 25 Years – 75 Years
• Healthy volunteers: Accepted

Summary

The present study was designed to assess the responsiveness of the hypothalamic pituitary gonadal axis to kisspeptin administration with increasing age in men.

Detailed description

Little is known about the involvement of kisspeptin signalling in the causation of senescence-basal modulation of the GnRH-LH unit in any model and particularly in advance age men. The present study was therefore designed to assess the response of hypothalamic pituitary gonadal axis to kisspeptin administration with increasing age in healthy men. Sequential blood samples (2 ml) were obtained for 30 minutes pre and 120 minutes post-kisspeptin injection periods at 30 min intervals (-30, 0, 30, 60, 90, 120). Kisspeptin-10 was administered (1 µg/kg BW) as an intravenous bolus, immediately after collecting 0 min sample to assess the response of GnRH neurons to kisspeptin-10 with increasing age in men by determining plasma levels of LH and testosterone.

Conditions

• Sterility, Reproductive

Interventions

Type	Name	Description
BIOLOGICAL	Human kisspeptin-10 (metastin 45-54)	Kisspeptin-10 (metastin 45-54, Calbiochem, Darmstadt, Germany) is a potent HPG-axis stimulator and control reproduction.

Timeline

• Start date: 2014-08-08
• Primary completion: 2014-08-10
• Completion: 2015-03-05
• First posted: 2017-10-20
• Last updated: 2017-10-23

Locations

1 site across 1 country: Pakistan