NCT03305510

Vitamin D Deficiency of Couples and Infertility

Prospective Cohort Study About Vitamin D Deficiency of Couples and Infertility

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 1,500 (actual)
• Sponsor: Tongji Hospital · Academic / Other
• Sex: All
• Age: 20 Years – 42 Years
• Healthy volunteers: Not accepted

Summary

Between March 2016 and March 2017, we performed a large retrospective, non-interventional, single-center cohort study and measured 25(OH)D3 plasma levels prior to ovarian stimulation in couples who underwent IVF/ICSI.And we analysed the correlation of vitamin D status and reproductive capacity .

Detailed description

Before conducting ovarian stimulation, all couples must conduct preoperative routine examination such as routine blood test, blood group examination and infectious disease examination. We collected 2ml remaining blood from the examination by use of sodium heparin, which then conducted 1,25(OH)2D3 assay by liquid chromatography-tandem mass spectrometry. All patients underwent a standardized ovarian stimulation regimen, oocyte retrieval, and fertilization, followed by a planned fresh or frozen embryo transfer.Normal fertilization rate was defined as the presence of two pronuclei (2PN) at the time of fertilization assessment, 16-19 h after ICSI or conventional insemination. The percentage of top quality embryos was defined as the percentage of top quality embryos among the total number of 2PN embryos. Blastocyst forming rate was calculated as number of formed blastocysts devided by number of cultured blastocysts.

Conditions

• Vitamin D Deficiency
• Infertility
• Couples
• In-vitro Fertilization

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	vitamin D is below 20ng/ml

Timeline

• Start date: 2016-03-01
• Primary completion: 2017-03-01
• Completion: 2017-06-01
• First posted: 2017-10-10
• Last updated: 2017-10-10