NCT03256370

Association of HMOs and Allergic Manifestations

The Association Between Human Milk Oligosaccharide and Development of Allergic Manifestations in Infants

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 150 (estimated)
• Sponsor: Chulalongkorn University · Academic / Other
• Sex: All
• Age: 2 Years
• Healthy volunteers: Accepted

Summary

Compare the HMO composition in breast milk of infants with allergic manifestation (atopic dermatitis and food allergy) in the first 3 years of life, healthy infants with allergic mother and healthy infants with non-allergic mother.

Detailed description

* Parents of breast-fed infant from general pediatric clinic and allergy clinic of King Chulalongkorn Memorial Hospital were approached for informed about the research and ask to join this project * After completed informed consent, demographic data was collected and filled in case record form * Milk of mother in both allergic and control groups were collected 60 ml then transferred to freeze at -80oc to for human milk oligosaccharide analysis

Conditions

• Human Milk Oligosaccharides, Allergic Manifestation

Interventions

Type	Name	Description
PROCEDURE	milk collection	* Parents of breast-fed infant from general pediatric clinic and allergy clinic of King Chulalongkorn Memorial Hospital were approached for informed about the research and ask to join this project * After completed informed consent, demographic data was collected and filled in case record form * Milk of mother in both allergic and control groups were collected 60 ml then transferred to freeze at -80oc to for human milk oligosaccharide analysis Method of human milk oligosaccharide analysis : 1. Ultra High Pressure Liquid Chromatography(UHPLC) with fluorescence method 2. High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) method

Timeline

• Start date: 2017-11-01
• Primary completion: 2018-10-05
• Completion: 2019-10-05
• First posted: 2017-08-22
• Last updated: 2017-10-17

Locations

1 site across 1 country: Thailand