NCT03189316

Anti-inflammatory Cells in Peritoneal Cavity

Can the Peritoneal Cavity be a Source of Regulatory B Cells to Treat Auto-immune Disease?

• Status: Terminated
• Phase: N/A
• Study type: Interventional
• Enrollment: 10 (actual)
• Sponsor: University Hospital, Montpellier · Academic / Other
• Sex: All
• Age: 18 Years – 80 Years
• Healthy volunteers: Not accepted

Summary

Some subsets of lymphocytes are able to inhibit immune response and thus, could be used to control auto-immune diseases and transplant reject. In mice, the main source of those anti-inflammatory lymphocytes is the peritoneal cavity. No data are available in human. This study aims at exploring the presence of those anti-inflammatory lymphocytes in human peritoneal cavity and at determine how to expand those cells.

Detailed description

B cells will be collected during procedures planned for patients' standard care. Peritoneal fluid and peripheral blood of patients will be collected during coelioscopy for exploration of ovarian cysts or dialysis for treatment of chronic renal insufficiency. Regulatory B cells (Breg cells) will be measured in peritoneal fluid and in peripheral blood after in vitro stimulation and assessment of intracellular interleukin-10 by flow cytometry. Proportion of Breg cells among B cells will be compared between peritoneal fluid and peripheral blood in both groups. Different protocols to induce differentiation of B cells into Breg cells will be tested and compared.

Conditions

• Ovarian Cysts
• Chronic Renal Insufficiency

Interventions

Type	Name	Description
OTHER	peritoneal fluid and blood sample	Peritoneal fluid sample obtained from peritoneal dialysis fluid of patients with chronic renal insufficiency and blood sample or Peritoneal fluid obtained from coelioscopy for exploration of ovarian cysts and blood cell

Timeline

• Start date: 2017-06-06
• Primary completion: 2017-12-13
• Completion: 2017-12-13
• First posted: 2017-06-16
• Last updated: 2018-06-13

Locations

1 site across 1 country: France