NCT03121924

Effect of Timing of Oocyte Denudation Before ICSI in an Oocyte Donation Model

Effect of Timing of Oocyte Denudation Before ICSI and Its Results in an Oocyte Donation Model: a Cluster Randomized Controlled Clinical Trial

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 375 (actual)
• Sponsor: Hospital Italiano de Buenos Aires · Academic / Other
• Sex: Female
• Age: 18 Years – 32 Years
• Healthy volunteers: Accepted

Summary

Analyze the effect of preincubation time of oocyte on the results of ICSI in a oocyte donation model.

Detailed description

There is an intense communication inside the follicular structure. Bidirectional information between the oocyte and the granulosa cells is needed for a normal follicular development and oocyte competence. There are conflicting results on the effect of pre-incubation time in ICSI. It is not clear the proper time to denudate the oocyte from the somatic cells prior to ICSI, and some authors describe a negative impact of the premature cummulus removal on the ICSI results. The aim of this study is to investigate wheter a preincubation time between oocyte retireval and injection has any effect on the ICSI ( intracytoplasmatic sperm injection) results .

Conditions

• Fertility

Interventions

Type	Name	Description
PROCEDURE	timing for oocyte denudation and Intracytoplasmatic Sperm injection	Donated oocytes were randomly allocated to two groups concerning the time of cumulus corona removal. The first group: After oocyte retrieval, cumulus cells were immediately removed and then ICSI was performed; in the second group, the oocyte-cumulus complexes were incubated for 4 hours. Afterwards, the granulosa cells were removedand ICSI was immediately performed.

Timeline

• Start date: 2015-12-01
• Primary completion: 2018-12-01
• Completion: 2019-08-01
• First posted: 2017-04-20
• Last updated: 2020-12-01

Locations

1 site across 1 country: Argentina