NCT03119350

Mitochondrial Energy Metabolism in Obese Women

Mitochondrial Energy Metabolism in Obese Women Undergoing Concurrent Physical Training

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 14 (actual)
• Sponsor: University of Sao Paulo · Academic / Other
• Sex: Female
• Age: 20 Years – 40 Years
• Healthy volunteers: Not accepted

Summary

Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses: 1. The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ; 2. The unbalance is therapeutically modified through physical training ; 3. Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program. This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.

Detailed description

Specific objectives: Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot. The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.

Conditions

• Metabolism Disorder
• Mitochondrial Alteration
• Physical Activity
• Obesity

Interventions

Type	Name	Description
OTHER	Physical Training	Intervention with concurrent physical training: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.

Timeline

• Start date: 2016-04-01
• Primary completion: 2016-07-01
• Completion: 2016-09-15
• First posted: 2017-04-18
• Last updated: 2019-04-30

Locations

1 site across 1 country: Brazil