NCT03062969

The Transition From Blastomere to Trophectoderm Biopsy: Comparing Two PGS Strategies

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 383 (actual)
• Sponsor: Fundacion Dexeus · Academic / Other
• Sex: Female
• Age: 18 Years – 45 Years
• Healthy volunteers: Not accepted

Summary

Shortly after the implementation of Comprehensive Chromosome Screening (CCS) techniques for Preimplantation Genetic Screening (PGS) came the transition in biopsy timing. Trophectoderm biopsy is meant to overcome the limitations of cleavage stage biopsy and single cell analysis.

Detailed description

The investigators have recently introduced this new approach and a frozen-thawed policy of euploid blastocysts is routinely performed. This is a single centre retrospective study in patients undergoing a PGS cycle. The aim of the study is to assess the results obtained in a PGS programme after the implementation of a new biopsy timing and transfer policy. Results obtained in PGS cycles with blastocyst biopsy and frozen-thawed embryo transfer were analysed in comparison to cycles with cleavage stage biopsy and fresh blastocyst transfer.

Conditions

• Infertility
• Preimplantation Genetic Screening

Interventions

Type	Name	Description
PROCEDURE	Cleavage stage biopsy group	* intracytoplasmatic sperm injection (ICSI)- (Invitro Fertilization)IVF * Time lapse embryo culture * Cleavage stage biopsy * aCGH analysis * Fresh euploid embryo transfer
PROCEDURE	Trophectoderm biopsy group	* ICSI-IVF * Time lapse embryo culture * Day 3 zona pellucida opening * Trophectoderm biopsy * aCGH analysis * Frozen-thawed euploid embryo transfer

Timeline

• Start date: 2014-01-01
• Primary completion: 2016-02-28
• Completion: 2017-01-01
• First posted: 2017-02-24
• Last updated: 2017-02-24