NCT02930031

Redox Status and Immune Function

Evidence of a Redox-dependent Regulation of Immune Responses to Exercise-Induced Inflammation

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 10 (actual)
• Sponsor: National and Kapodistrian University of Athens · Academic / Other
• Sex: Male
• Age: 18 Years – 30 Years
• Healthy volunteers: Accepted

Summary

In this investigation the investigators utilized N-acetylcysteine (NAC) supplementation to enhance reduced glutathione (GSH) stores during an 8-day recovery period from a strenuous eccentric exercise protocol in order to test the hypotheses: i) redox status perturbations in skeletal muscle are pivotal for the immune responses and ii) antioxidant supplementation may alter immune cell responses following exercise-induced muscle microtrauma.

Detailed description

The major thiol-disulfide couple of GSH and oxidized glutathione (GSSG) is a crucial regulator of the main transcriptional pathways regulating aseptic inflammation and recovery of skeletal muscle following aseptic injury. Antioxidant supplementation may hamper exercise-induced inflammatory responses. The objective was to examine how thiol-based antioxidant supplementation affects immune mobilization following exercise-induced skeletal muscle microtrauma. In a two-trial, double-blind, crossover, repeated measures design, 10 young men received either placebo or NAC (20 mg/kg/day) immediately after a muscle-damaging exercise protocol (300 eccentric contractions) and for eight consecutive days. Blood sampling and performance assessment were performed pre-exercise, 2h post-exercise and daily for 8 consecutive days.

Conditions

• Skeletal Muscle Damage
• Aseptic Inflammation
• Skeletal Muscle Function

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	n-acetylcysteine	20 mg//kg/day, orally, daily for eight days following exercise
DIETARY_SUPPLEMENT	Placebo	500 mL orally, daily for eight days following exercise

Timeline

• Start date: 2015-01-01
• Primary completion: 2016-02-01
• Completion: 2016-03-01
• First posted: 2016-10-11
• Last updated: 2016-10-11

Locations

1 site across 1 country: Greece